The Forum provides a unique opportunity to discuss advances in the next-generation of eco-sustainable CO2 Separation (SFC) and Extraction (SFE) technologies. PIC Solution brings together SFC thought leaders to demonstrate the critical roles of SFC and SFE in the isolation of chiral/achiral Small Molecules, Peptides and Natural Products including Cannabis/Hemp. Technology enhancements to optimize instruments performance, increase throughput, reduce costs and minimize environmental impact will be also covered.
8.30 am Mengling Wong, Genentech Inc.
Separation of chiral compounds via SFC to support Genentech’s Drug Discovery Programs
Many molecules are developed and evaluated during early stages of drug discovery. The objective of Medicinal chemists is to design and synthesize molecules that will not only bind with high affinity to the target protein active site but also have good physicochemical properties. To achieve this objective, these molecules will often have one or more stereo-centers and the pure stereoisomer of interest will need to be isolated for testing, development, and regulatory approval. Genentech’s Analytical Research group has developed an optimized chiral screening workflow that utilizes a set of 15 chiral phases. This chiral screen uses short columns, short run times, and high flow rates to achieve rapid, high quality results. This approach has enabled us to rapidly screen and then scale-up to purify milligram to multi-gram quantities of chiral molecules in support of our drug discovery programs.
9.00 am Antoni Severino, Ph.D., UCB Pharma
SFC support to Medicinal Chemistry and Chemical Process Development from mg to kg
To support Medicinal Chemistry and New Process Development projects in Pharmaceutical R&D, day after day, numerous intermediates or potential Active Pharmaceutical Ingredient (API) candidates are synthesized. The chemistry and isolation steps are rarely optimized and chromatographic purification steps are needed to timely delivered the compounds of interest. In UCB the well-established liquid purifications are performed for almost 30 years. At a prep scale, Reverse Phase (RP) on C18 or Normal Phase (NP) on silica are generally made based on the amount to purify, on the compound’s polarity, and taking into account chemistry’s constraints. Over the past 10 years, the SFC purifications became more and more considered and applied to get orthogonality with HPLC, to perform 24/7 process for very large scale and to decrease the amount of solvent to evaporate. This talk will be the opportunity to consider the strategy developed over the years to quickly purify different type of samples by choosing the right stationary phase for the right purpose or by choosing between HPLC and SFC. With regards to the chiral separations, some study cases will be presented to highlight the importance of the columns screening, the solubility of the samples and to compare SFC with Multicolumn Continuous (liquid) Chromatography process.
9.30 am Eric Kawka, Cattis LLC
Implementation of Supercritical Fluid Chromatography for the Concentration and Isolation of Cannabinoids from Hemp Extracts
The 2018 Farm Bill has opened many opportunities associated with the hemp plant. While cannabidiol (CBD) has shown promising health benefits, new opportunities to access minor cannabinoids have emerged. Minor cannabinoids such as Cannabigerol (CBG) and Cannabichromene (CBC) can be accessed directly from the plant through various breeding programs, while minor cannabinoids such as Cannabinol (CBN) are accessed through manipulation / degradation of CBD. As new cannabinoids like CBN emerge on the market, the responsibility of manufactures must increase in order to create safe products. Here we will discuss the utility of Supercritical Fluid Chromatography (SFC) for the isolation of hemp-derived cannabinoids.
10.00 am Bill Farrell, Pfizer Inc.
Leveraging Supercritical Fluid Chromatography for Some Unusual Application
This presentation will highlight developments in SFC technology that have opened new opportunities to impact Medicinal Chemistry projects. One example will describe how sample counterion equivalents can be quantitated rapidly with minimal sample usage using ELSD technology. Another application will demonstrate several unique characteristics of SFC chromatography, which enable the development of a methodology for measuring intra-molecular hydrogen bonding differences between matched pairs of compounds.
10.30 am Gerard Rosse, Ph.D., PIC Solution, Inc.
PIC Solution Latest SFC, SFC-MS and SFE Innovations
An overview of the novel technologies developed by PIC Solution with a focus on the newly designed Open-Bed SFC-MS and high-through analytical SFC systems (2-5 columns in parallel) will be discussed. The Open-Bed SFC-MS enhances the eco-friendly and sustainability benefits of SFC with CO2 recycling in Open-Bed mode and capabilities to conduct separation in the low mg scale.